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1. Field-EPSPs recording in brain slices
2. Whole-cell recording in brain slices
3. Whole-cell recording in culture neurons or cell lines
4. Calcium imaging recording
I. Instrument：
1. Olympus BX51W1 optical microscope (Main)
2. With Olympus XM10 Fluorescent microscope
3. With DP72 HQ CCD, Xenon lamp and Sony XC-EI50 IR-CCD
4. Computer-Controlled Dual Channel Resistive-Feedback Patch Clamp Amplifier
5. Axon MultiClamp 700B amplifier
6. Power 1401 mk II CED
7. 2 sets of LN 3 Axis dial controllers
8. Badcontroller V platform dual temperature controller
9. Olympus U-RFL-T halogen light source system
II. Function：
1. Whole-cell recording
2. Field-recording
I. Instrument：
1. DAGE-MTI IR-1000 CCD Infrared Image camera
2. Computer-Controlled Dual Channel Resistive-Feedback Patch Clamp Amplifier
3. Axon MultiClamp 700B amplifier
4. Power 1401 mk II CED
5. Automatic triming controller
- PS-7000C PatchStar Micromanipulator
- MST-1000 MicroStar Micromanipulator
6. TC-344B Heater controller
7. Triple port OptoLED System
II. Function：
1. Whole-cell recording
2. Field-recording
I. Instrument：
1. Calcium Imaging:
- Lamda 10-2 Optical Filter Changer with Fura-2, Indo-1, and standard Dapi, FITC, Rhodamine filter sets
- Perfusion and Temperature control system:
- VC-6 Six channel valve controller
- Inline and platform dual temperature controller
2. Software:
1. MetaFlour for fluorescence ratio imaging
2. MetaMorph
II. Function：
1. Calcium Imaging
I. Instrument：
1. Electrophysiology:
- Axonpatch 200B amplifier
- digidata 1440A digitizer
- axis dial controller
2. Software:
- pClamp 10 including Axoscope, Clampfit, and Cample
II. Function：
1. Whole-cell recording
2. single-cell microinjection
3. single-cell PCR
I. Others
1. Vibrotome – D.S.K. microslicer DTK-1000
2. Glass Pipette puller – NARISHIGE PC-10
3. Horizontal Pipette puller - SUTTER MODEL P-97
4. Micro-Osmometer Model 3300
II. Software：
- Spike2
- Signal Ver. 4.08
- Multiclamp 700B
- Multicam Studio
- cellSens Standard
- MetaFlour for fluorescence ratio imaging
- MetaMorph
- pClamp 10 including Axoscope, Clampfit, and Camplex
Lab Info
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