1. Using extracellular recording techniques recorded field EPSP (Field excitatory postsynaptic potential) in hippocampus slices. Placing the stimulating and recording electrodes can record the transmission signal of neural circuits. The hippocampus is traditional cerebral cortex, the arrangement and composition of nerve structure is more simply, so usually used as a nerve conduction study materials. The hippocampus has been identified that related with spatial learning and memory.Currently plan to open the service areas of the brain is the hippocampal CA1. Filling the glass electrode with 3M NaCl as the recording electrode placed in CA1 stratum radiatum, a bipolar stainless steel stimulating electrodes were placed to stimulate the Schaffer collateral branch in the vicinity of the electrodes record. Using different electrical stimulation conditions and drug administration can induced long-term potentiation (LTP) or long-term depression (LTD) in hippocampal CA1 synapse.Using the pharmacological,will enable researchers explore more carefully of the physiological response, and possible mechanism of drugs.
2. Patch clamp electrophysiology is to use a glass microelectrode to form a tight seal with cell membrane and is able to detect ions flow across cell membrane. It provides a powerful tool to investigate electrical properties of cells in unprecedented detail. In combination electrophysiology and other tools like molecular biology and pharmacology further provides great insight into mechanisms of biological processes.Intracellular calcium plays important role in many different physiological processes such as second messenger pathways, muscle contraction, ion channel gating, cell proliferation, apoptosis etc. Therefore, calcium quantification is performed in a variety of cellular studies to highlight molecular mechanism involved in different process. Calcium imaging with fluorescent indicators has become the most popular method. This method utilizes fluorescent probes that show a spectral response upon binding calcium and enables researchers to investigate changes in intracellular free calcium concentrationsThe electrophysiology and calcium imaging core facility provides the neuroscientists and other life science investigators in Academia Sinica with a facility to help generate electrophysiology and calcium ion information in cultured cells.